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产品目录

AhlI内切酶图片
产品货号:
GS2142
中文名称:
AhlI内切酶
英文名称:
AhlI
产品规格:
200U
发货周期:
1~3天
产品价格:
询价
储存条件:冷冻(-20℃)

使用说明

To obtain 100%activity,BSA should be added to the 1x reaction mix to a final concentration of 100μg/ml.Do not use BSA for long incubation

技术规格

Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of T7 DNA in 1hourat 37℃ in a total reaction volume of 50μl.
Recognition sitesA↑CTAGT
来源Alteromonas haloplanktis SP
Assayed onT7 DNA
Optimal bufferB(10mMTris-HCl(pH 7.6 at 25℃);10mMMgCl2;1 mM DTT.)+ BSA
Optimal temperature37℃
Storage conditions10mMTris-HCl(pH 7.5);100 mM KCl;0.1 mM EDTA;7 mM 2-mercaptoethanol;200 μg/ml BSA;50%glycerol;Store at -20℃.
LigationsAfter 20-fold overdigestion with enzyme more than 90%of T7 DNA fragments can be ligated and recut.
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1 μg of T7 DNA with 40 u.a.of enzyme for 16hoursat 37℃.
Methlation effectMethylation sensitivity not tested
Thermal inactivationNO
Campatible EndsBcuI,SpeI

相关搜索:AhlI内切酶AhlI限制性内切酶
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