产品货号：
GS2142
中文名称：
AhlI内切酶
英文名称：
AhlI
产品规格：
200U
发货周期：
1～3天
产品价格：
询价
储存条件：冷冻（-20℃）
使用说明
To obtain 100%activity,BSA should be added to the 1x reaction mix to a final concentration of 100μg/ml.Do not use BSA for long incubation
技术规格
相关搜索：AhlI内切酶，AhlI限制性内切酶
使用说明
To obtain 100%activity,BSA should be added to the 1x reaction mix to a final concentration of 100μg/ml.Do not use BSA for long incubation
技术规格
| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of T7 DNA in 1hourat 37℃ in a total reaction volume of 50μl. |
| Recognition sites | A↑CTAGT |
| 来源 | Alteromonas haloplanktis SP |
| Assayed on | T7 DNA |
| Optimal buffer | B(10mMTris-HCl(pH 7.6 at 25℃);10mMMgCl2;1 mM DTT.)+ BSA |
| Optimal temperature | 37℃ |
| Storage conditions | 10mMTris-HCl(pH 7.5);100 mM KCl;0.1 mM EDTA;7 mM 2-mercaptoethanol;200 μg/ml BSA;50%glycerol;Store at -20℃. |
| Ligations | After 20-fold overdigestion with enzyme more than 90%of T7 DNA fragments can be ligated and recut. |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of T7 DNA with 40 u.a.of enzyme for 16hoursat 37℃. |
| Methlation effect | Methylation sensitivity not tested |
| Thermal inactivation | NO |
| Campatible Ends | BcuI,SpeI |
相关搜索：AhlI内切酶，AhlI限制性内切酶